English: Microtubule disassembly interferes with Cav1-positive vesicle trafficking. Hela cells expressing Cav1-mRFP were kept in suspension for 1 h, and then replated on fibronectin-coated substrate for 3 h and further incubated in the presence of nocodazole for 30 min. Cells were visualized using time-lapse spinning disk microscopy. Images were taken every 2 s. Under Nocodazole treatment, Cav1- positive vesicles are static and concentrated in the cell center.